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Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.


ABSTRACT: Over the past few decades, nucleic acid-based methods have been developed for the diagnosis of intestinal parasitic infections. Advantages of nucleic acid-based methods are numerous; typically, these include increased sensitivity and specificity and simpler standardization of diagnostic procedures. DNA samples can also be stored and used for genetic characterization and molecular typing, providing a valuable tool for surveys and surveillance studies. A variety of technologies have been applied, and some specific and general pitfalls and limitations have been identified. This review provides an overview of the multitude of methods that have been reported for the detection of intestinal parasites and offers some guidance in applying these methods in the clinical laboratory and in epidemiological studies.

SUBMITTER: Verweij JJ 

PROVIDER: S-EPMC3993103 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.

Verweij Jaco J JJ   Stensvold C Rune CR  

Clinical microbiology reviews 20140401 2


Over the past few decades, nucleic acid-based methods have been developed for the diagnosis of intestinal parasitic infections. Advantages of nucleic acid-based methods are numerous; typically, these include increased sensitivity and specificity and simpler standardization of diagnostic procedures. DNA samples can also be stored and used for genetic characterization and molecular typing, providing a valuable tool for surveys and surveillance studies. A variety of technologies have been applied,  ...[more]

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