Ontology highlight
ABSTRACT:
SUBMITTER: Thiel A
PROVIDER: S-EPMC3993132 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Thiel Axel A Michoud Grégoire G Moalic Yann Y Flament Didier D Jebbar Mohamed M
Applied and environmental microbiology 20140131 7
In this study, we developed a gene disruption system for Thermococcus barophilus using simvastatin for positive selection and 5-fluoroorotic acid (5-FOA) for negative selection or counterselection to obtain markerless deletion mutants using single- and double-crossover events. Disruption plasmids carrying flanking regions of each targeted gene were constructed and introduced by transformation into wild-type T. barophilus MP cells. Initially, a pyrF deletion mutant was obtained as a starting poin ...[more]