Identification of CMY-2-type cephalosporinases in clinical isolates of Enterobacteriaceae by MALDI-TOF MS.
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ABSTRACT:
SUBMITTER: Papagiannitsis CC
PROVIDER: S-EPMC3993231 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Publications
Identification of CMY-2-type cephalosporinases in clinical isolates of Enterobacteriaceae by MALDI-TOF MS.
Papagiannitsis C C CC Kotsakis S D SD Tuma Z Z Gniadkowski M M Miriagou V V Hrabak J J
Antimicrobial agents and chemotherapy 20140224 5
This study exploited the possibility to detect Citrobacter freundii-derived CMY-2-like cephalosporinases in Enterobacteriaceae clinical isolates using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Periplasmic proteins were prepared using a modified sucrose method and analyzed by MALDI-TOF MS. A ca. 39,850-m/z peak, confirmed to represent a C. freundii-like β-lactamase by in-gel tryptic digestion followed by MALDI-TOF/TOF MS, was observed only in CMY ...[more]
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