Ontology highlight
ABSTRACT:
SUBMITTER: Podany AT
PROVIDER: S-EPMC3993270 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Podany Anthony T AT Winchester Lee C LC Robbins Brian L BL Fletcher Courtney V CV
Antimicrobial agents and chemotherapy 20140310 5
An ultrasensitive assay utilizing high-pressure liquid chromatography and mass spectrometry detection was developed and validated for the quantification of the antiretrovirals atazanavir (ATV), darunavir (DRV), lopinavir (LPV), ritonavir (RTV), and efavirenz (EFV) in human mononuclear cell (MNC) extracts. The assay utilizes 20 μl of cellular extract that contains as few as 50,000 MNCs. The analytical range of the assay is 0.0200 to 10.0 fmol/μl for ATV, 0.0500 to 25.0 fmol/μl for DRV, LPV, and R ...[more]