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Establishment of intestinal microbiota during early life: a longitudinal, explorative study of a large cohort of Danish infants.

ABSTRACT: Fecal samples were obtained from a cohort of 330 healthy Danish infants at 9, 18, and 36 months after birth, enabling characterization of interbacterial relationships by use of quantitative PCR targeting 31 selected bacterial 16S rRNA gene targets representing different phylogenetic levels. Nutritional parameters and measures of growth and body composition were determined and investigated in relation to the observed development in microbiota composition. We found that significant changes in the gut microbiota occurred, particularly from age 9 to 18 months, when cessation of breastfeeding and introduction of a complementary feeding induce replacement of a microbiota characterized by lactobacilli, bifidobacteria, and Enterobacteriaceae with a microbiota dominated by Clostridium spp. and Bacteroides spp. Classification of samples by a proxy enterotype based on the relative levels of Bacteroides spp. and Prevotella spp. showed that enterotype establishment occurs between 9 and 36 months. Thirty percent of the individuals shifted enterotype between 18 and 36 months. The composition of the microbiota was most pronouncedly influenced by the time of cessation of breastfeeding. From 9 to 18 months, a positive correlation was observed between the increase in body mass index and the increase of the short-chain-fatty-acid-producing clostridia, the Clostridum leptum group, and Eubacterium hallii. Considering previously established positive associations between rapid infant weight gain, early breastfeeding discontinuation, and later-life obesity, the corresponding microbial findings seen here warrant attention.

SUBMITTER: Bergstrom A

PROVIDER: S-EPMC3993305 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Establishment of intestinal microbiota during early life: a longitudinal, explorative study of a large cohort of Danish infants.

Bergström Anders A Skov Thomas Hjort TH Bahl Martin Iain MI Roager Henrik Munch HM Christensen Line Brinch LB Ejlerskov Katrine Tschentscher KT Mølgaard Christian C Michaelsen Kim F KF Licht Tine Rask TR

Applied and environmental microbiology 20140228 9

Fecal samples were obtained from a cohort of 330 healthy Danish infants at 9, 18, and 36 months after birth, enabling characterization of interbacterial relationships by use of quantitative PCR targeting 31 selected bacterial 16S rRNA gene targets representing different phylogenetic levels. Nutritional parameters and measures of growth and body composition were determined and investigated in relation to the observed development in microbiota composition. We found that significant changes in the ...[more]

PMID: 24584251

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Project description:PURPOSE:The Danish Work Life Course Cohort (DaWCo) was initiated to study relations between working conditions, health and labour market affiliation using repeated measures of these factors throughout the working life, while accounting for health-differences pre-existing labour market entry. PARTICIPANTS:The cohort encompasses all 15-30-year-old individuals residing in Denmark who entered the labour market during the years 1995-2009 (960 562 individuals and 7 136 188 observations). Data include information on working conditions measured by job exposure matrices linked with registers on health, labour market affiliation and sociodemographics for both the cohort members and their parents. The median age at cohort entry was 20 years and men and women were equally represented. FINDINGS TO DATE:Currently, one study has been published, which found that low job control was associated with increased risk of depressive disorder, independently from indicators of socioeconomic position measured throughout the life-course. The present cohort profile presents data regarding the transitions of cohort members between states of labour market affiliation and data on health services use. All cohort members were employed in their year of entry, but this proportion decreased across the years to 82.4% in the 10th year since cohort entry. The proportion of students peaked at 5 years since cohort entry with 13.9%. FUTURE PLANS:This large prospective cohort offers the possibility to study associations between psychosocial working conditions and rare outcomes and to examine the potential accumulation of effects while accounting for health-differences pre-existing labour market entry. Currently, we are working on analyses on risk of hospital-diagnosed incident depression and disability pensioning. The study is ongoing, and we are planning to extend the study to include the years 2010-2018 and expand the cohort with individuals entering the Danish workforce during these years.

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Dataset Information

Establishment of intestinal microbiota during early life: a longitudinal, explorative study of a large cohort of Danish infants.

Publications

Establishment of intestinal microbiota during early life: a longitudinal, explorative study of a large cohort of Danish infants.

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