Simultaneous detection of five enteric viruses associated with gastroenteritis by use of a PCR assay: a single real-time multiplex reaction and its clinical application.
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ABSTRACT:
SUBMITTER: Jiang Y
PROVIDER: S-EPMC3993471 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
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Simultaneous detection of five enteric viruses associated with gastroenteritis by use of a PCR assay: a single real-time multiplex reaction and its clinical application.
Jiang Yixiang Y Fang Lin L Shi Xiaolu X Zhang Hailong H Li Yinghui Y Lin Yiman Y Qiu Yaqun Y Chen Qingliang Q Li Hui H Zhou Li L Hu Qinghua Q
Journal of clinical microbiology 20140129 4
We developed a highly sensitive reverse transcription and multiplex real-time PCR (rtPCR) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups I and II; sapovirus genogroups I, II, IV, and V; human rotavirus A; adenovirus serotypes 40 and 41; and human astrovirus. In comparison to monoplex rtPCR assays, the sensitivities and specificities of the multiplex rtPCR ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clini ...[more]
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