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Rapid detection of ?-lactamase-hydrolyzing extended-spectrum cephalosporins in Enterobacteriaceae by use of the new chromogenic ?Lacta test.

ABSTRACT: The chromogenic ?Lacta test developed for the rapid detection of ?-lactamase-hydrolyzing extended-spectrum cephalosporins in Enterobacteriaceae revealed good performance with extended-spectrum ?-lactamase (ESBL) producers (97.5% true-positive results). However, false-negative results occurred with chromosomal AmpC hyperproducers and plasmid AmpC producers, whereas uninterpretable results were mostly due to VIM-1 carbapenemase producers and possibly low levels of expressed ESBLs.

SUBMITTER: Morosini MI

PROVIDER: S-EPMC3993668 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Rapid detection of β-lactamase-hydrolyzing extended-spectrum cephalosporins in Enterobacteriaceae by use of the new chromogenic βLacta test.

Morosini María Isabel MI García-Castillo María M Tato Marta M Gijón Desirèe D Valverde Aránzazu A Ruiz-Garbajosa Patricia P Cantón Rafael R

Journal of clinical microbiology 20140226 5

The chromogenic βLacta test developed for the rapid detection of β-lactamase-hydrolyzing extended-spectrum cephalosporins in Enterobacteriaceae revealed good performance with extended-spectrum β-lactamase (ESBL) producers (97.5% true-positive results). However, false-negative results occurred with chromosomal AmpC hyperproducers and plasmid AmpC producers, whereas uninterpretable results were mostly due to VIM-1 carbapenemase producers and possibly low levels of expressed ESBLs. ...[more]

PMID: 24574293

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