Identification and differentiation of Candida parapsilosis complex species by use of exon-primed intron-crossing PCR.
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ABSTRACT:
SUBMITTER: Feng X
PROVIDER: S-EPMC3993672 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
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Identification and differentiation of Candida parapsilosis complex species by use of exon-primed intron-crossing PCR.
Feng Xiaobo X Wu Zengbin Z Ling Bo B Pan Shuming S Liao Wanqing W Pan Weihua W Yao Zhirong Z
Journal of clinical microbiology 20140312 5
The Candida parapsilosis complex is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis, Candida metapsilosis, and the closely related species Lodderomyces elongisporus. An exon-primed intron-crossing PCR assay was developed here to distinguish the members of the species complex on the basis of the distinct sizes of amplicons, and Candida orthopsilosis and Candida metapsilosis were further discriminated by restriction enzyme analysis. ...[more]
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