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Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype isolates.


ABSTRACT: Mycobacterium tuberculosis Beijing genotype strains are rapidly disseminating, frequently hypervirulent, and multidrug resistant. Here, we describe a method for their rapid detection by real-time PCR that targets the specific IS6110 insertion in the dnaA-dnaN genome region. The method was evaluated with a geographically and genetically diverse collection representing areas in East Asia and the former Soviet Union in which the Beijing genotype is endemic and epidemic (i.e., major foci of its global propagation) and with clinical specimens.

SUBMITTER: Mokrousov I 

PROVIDER: S-EPMC3993684 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype isolates.

Mokrousov Igor I   Vyazovaya Anna A   Zhuravlev Viacheslav V   Otten Tatiana T   Millet Julie J   Jiao Wei-Wei WW   Shen A-Dong AD   Rastogi Nalin N   Vishnevsky Boris B   Narvskaya Olga O  

Journal of clinical microbiology 20140212 5


Mycobacterium tuberculosis Beijing genotype strains are rapidly disseminating, frequently hypervirulent, and multidrug resistant. Here, we describe a method for their rapid detection by real-time PCR that targets the specific IS6110 insertion in the dnaA-dnaN genome region. The method was evaluated with a geographically and genetically diverse collection representing areas in East Asia and the former Soviet Union in which the Beijing genotype is endemic and epidemic (i.e., major foci of its glob  ...[more]

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