High-affinity recombinant antibody fragments (Fabs) can be applied in peptide enrichment immuno-MRM assays.

ABSTRACT: High-affinity antibodies binding to linear peptides in solution are a prerequisite for performing immuno-MRM, an emerging technology for protein quantitation with high precision and specificity using peptide immunoaffinity enrichment coupled to stable isotope dilution and targeted mass spectrometry. Recombinant antibodies can be generated from appropriate libraries in high-throughput in an automated laboratory and thus may offer advantages over conventional monoclonal antibodies. However, recombinant antibodies are typically obtained as fragments (Fab or scFv) expressed from E. coli, and it is not known whether these antibody formats are compatible with the established protocols and whether the affinities necessary for immunocapture of small linear peptides can be achieved with this techno

SUBMITTER: Whiteaker JR 

PROVIDER: S-EPMC3993957 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature