Purification of native Arp2/3 complex from bovine thymus.
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ABSTRACT:
SUBMITTER: Doolittle LK
PROVIDER: S-EPMC3994882 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Purification of native Arp2/3 complex from bovine thymus.
Doolittle Lynda K LK Rosen Michael K MK Padrick Shae B SB
Methods in molecular biology (Clifton, N.J.) 20130101
The Arp2/3 complex is an actin filament nucleator involved in cell motility and vesicle trafficking. Owing to the role the complex plays in important and fundamental cell biological processes, the purified complex is used in biochemical assays, reconstituted motility assays, and structural biology. As this is a eukaryotic complex assembled from seven polypeptides, the complex is purified from eukaryotic sources. Described here is a detailed method for purification of the complex from a mammalian ...[more]
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