Ontology highlight
ABSTRACT:
SUBMITTER: Kakiyama G
PROVIDER: S-EPMC3995475 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Kakiyama Genta G Muto Akina A Takei Hajime H Nittono Hiroshi H Murai Tsuyoshi T Kurosawa Takao T Hofmann Alan F AF Pandak William M WM Bajaj Jasmohan S JS
Journal of lipid research 20140313 5
We have developed a simple and accurate HPLC method for measurement of fecal bile acids using phenacyl derivatives of unconjugated bile acids, and applied it to the measurement of fecal bile acids in cirrhotic patients. The HPLC method has the following steps: 1) lyophilization of the stool sample; 2) reconstitution in buffer and enzymatic deconjugation using cholylglycine hydrolase/sulfatase; 3) incubation with 0.1 N NaOH in 50% isopropanol at 60°C to hydrolyze esterified bile acids; 4) extract ...[more]