Dataset Information

Polymorphic patterns of the merozoite surface protein-3? in Korean isolates of Plasmodium vivax.

ABSTRACT: BACKGROUND: The merozoite surface protein-3? of Plasmodium vivax (PvMSP-3?) is one of the candidate antigens for blood stage malaria vaccine development. The polymorphisms in PvMSP-3? have been reported in certain P. vivax isolates. However, the diversity of PvMSP-3? throughout its global distribution has not been well understood. In this study, the genetic diversity and the effects of natural selection in PvMSP-3? among P. vivax Korean isolates were analysed. METHODS: Blood samples were collected from 95 patients with vivax malaria in Korea. The region flanking full-length PvMSP-3? was amplified by polymerase chain reaction and cloned into a TA cloning vector. The PvMSP-3? sequence of each isolate was determined and the polymorphic characteristics and effects of natural selection were analysed using the DNASTAR, MEGA4, and DnaSP programs. RESULTS: Five different subtypes of PvMSP-3? were identified based on single nucleotide polymorphisms (SNPs), insertions, and deletions. Although a high level of sequence diversity was observed in the PvMSP-3? gene, the coiled-coil tertiary structure of the PvMSP-3? protein was well conserved in all of the sequences. The PvMSP-3? of Korean isolates is under natural selection. DNA polymerase slippage and intragenic recombination likely contributed to PvMSP-3? diversity in Korean P. vivax isolates. CONCLUSIONS: The PvMSP-3? of Korean P. vivax isolates displayed polymorphisms, with SNPs, insertions and deletions scattered throughout of the gene. These results of parasite heterogeneity are relevant to the development of a PvMSP-3? based vaccine against P. vivax and the implementation of malaria control programmes in Korea.

SUBMITTER: Kang JM

PROVIDER: S-EPMC3995521 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Publications

Polymorphic patterns of the merozoite surface protein-3β in Korean isolates of Plasmodium vivax.

Kang Jung-Mi JM Ju Hye-Lim HL Cho Pyo Yun PY Moon Sung-Ung SU Ahn Seong Kyu SK Sohn Woon-Mok WM Lee Hyeong-Woo HW Kim Tong-Soo TS Na Byoung-Kuk BK

Malaria journal 20140317

<h4>Background</h4>The merozoite surface protein-3β of Plasmodium vivax (PvMSP-3β) is one of the candidate antigens for blood stage malaria vaccine development. The polymorphisms in PvMSP-3β have been reported in certain P. vivax isolates. However, the diversity of PvMSP-3β throughout its global distribution has not been well understood. In this study, the genetic diversity and the effects of natural selection in PvMSP-3β among P. vivax Korean isolates were analysed.<h4>Methods</h4>Blood samples ...[more]

PMID: 24635878

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Project description:BackgroundMalaria control efforts have a significant impact on the epidemiology and parasite population dynamics. In countries aiming for malaria elimination, malaria transmission may be restricted to limited transmission hot spots, where parasite populations may be isolated from each other and experience different selection forces. Here we aim to examine the Plasmodium vivax population divergence in geographically isolated transmission zones in Thailand.MethodologyWe employed the P. vivax merozoite surface protein 3β (PvMSP3β) as a molecular marker for characterizing P. vivax populations based on the extensive diversity of this gene in Southeast Asian parasite populations. To examine two parasite populations with different transmission levels in Thailand, we obtained 45 P. vivax isolates from Tak Province, northwestern Thailand, where the annual parasite incidence (API) was more than 2%, and 28 isolates from Yala and Narathiwat Provinces, southern Thailand, where the API was less than 0.02%. We sequenced the PvMSP3β gene and examined its genetic diversity and molecular evolution between the parasite populations.Principal findingsOf 58 isolates containing single PvMSP3β alleles, 31 sequence types were identified. The overall haplotype diversity was 0.77 ± 0.06 and nucleotide diversity 0.0877±0.0054. The northwestern vivax malaria population exhibited extensive haplotype diversity (HD) of PvMSP3β (HD=1.0). In contrast, the southern parasite population displayed a single PvMSP3β allele (HD=0), suggesting a clonal population expansion. This result revealed that the extent of allelic diversity in P. vivax populations in Thailand varies among endemic areas.ConclusionMalaria parasite populations in a given region may vary significantly in genetic diversity, which may be the result of control and influenced by the magnitude of malaria transmission intensity. This is an issue that should be taken into account for the implementation of P. vivax control measures such as drug policy and vaccine development.

Dataset Information

Polymorphic patterns of the merozoite surface protein-3? in Korean isolates of Plasmodium vivax.

Publications

Polymorphic patterns of the merozoite surface protein-3β in Korean isolates of Plasmodium vivax.

Similar Datasets

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