Ontology highlight
ABSTRACT:
SUBMITTER: Rogstad SM
PROVIDER: S-EPMC3995537 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Rogstad Sarah M SM Sorkina Tatiana T Sorkin Alexander A Wu Christine C CC
Analytical chemistry 20130416 9
Mass spectrometry coupled immunoprecipitation (MS-IP) studies are useful in identifying and quantitating potential binding partners of a target protein. However, they are often conducted without appropriate loading controls. Western blots are often used to analyze loading controls, yet there are limitations to their usefulness as analytical tools. One remedy for this is the use of selected reaction monitoring (SRM), where the areas under the curve (AUCs) of peptides from a protein of interest ca ...[more]