Ontology highlight
ABSTRACT:
SUBMITTER: Watanabe T
PROVIDER: S-EPMC3996463 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Watanabe Tsunamasa T Hatakeyama Hiroto H Matsuda-Yasui Chiho C Sato Yusuke Y Sudoh Masayuki M Takagi Asako A Hirata Yuichi Y Ohtsuki Takahiro T Arai Masaaki M Inoue Kazuaki K Harashima Hideyoshi H Kohara Michinori M
Scientific reports 20140423
The development of RNA interference (RNAi)-based therapy faces two major obstacles: selecting small interfering RNA (siRNA) sequences with strong activity, and identifying a carrier that allows efficient delivery to target organs. Additionally, conservative region at nucleotide level must be targeted for RNAi in applying to virus because hepatitis C virus (HCV) could escape from therapeutic pressure with genome mutations. In vitro preparation of Dicer-generated siRNAs targeting a conserved, high ...[more]