Ontology highlight
ABSTRACT:
SUBMITTER: Saka SK
PROVIDER: S-EPMC3996535 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Saka Sinem K SK Vogts Angela A Kröhnert Katharina K Hillion François F Rizzoli Silvio O SO Wessels Johannes T JT
Nature communications 20140410
The isotopic composition of different materials can be imaged by secondary ion mass spectrometry. In biology, this method is mainly used to study cellular metabolism and turnover, by pulsing the cells with marker molecules such as amino acids labelled with stable isotopes ((15)N, (13)C). The incorporation of the markers is then imaged with a lateral resolution that can surpass 100 nm. However, secondary ion mass spectrometry cannot identify specific subcellular structures like organelles, and ne ...[more]