Ontology highlight
ABSTRACT:
SUBMITTER: Zheng F
PROVIDER: S-EPMC3996704 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Zheng Fang F Xue Liu L Hou Shurong S Liu Junjun J Zhan Max M Yang Wenchao W Zhan Chang-Guo CG
Nature communications 20140318
Compared with naturally occurring enzymes, computationally designed enzymes are usually much less efficient, with their catalytic activities being more than six orders of magnitude below the diffusion limit. Here we use a two-step computational design approach, combined with experimental work, to design a highly efficient cocaine hydrolysing enzyme. We engineer E30-6 from human butyrylcholinesterase (BChE), which is specific for cocaine hydrolysis, and obtain a much higher catalytic efficiency f ...[more]