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Expression of SDF-1 and CXCR4 transcript variants and CXCR7 in epithelial ovarian cancer.


ABSTRACT: Chemokine stromal cell-derived factor-1 (SDF-1) and its receptors, CXCR4 and CXCR7, have been implicated in epithelial ovarian cancer progression and metastasis. However, limited data are available on the expression levels of SDF-1 and CXCR4 variants and CXCR7 in human epithelial ovarian cancer. The present study aimed to characterize the expression pattern and levels of SDF-1, CXCR4 and CXCR7 in normal human ovaries and epithelial ovarian cancer. The expression of SDF-1 and CXCR4 transcript variants and CXCR7 was determined by quantitative polymerase chain reaction (qPCR). Plasma SDF-1? levels were determined by commercially available EIA kits and cancer antigen 125 (CA 125) levels were quantified by automated microparticle enzyme immunosorbent assay. High expression levels of SDF-1 transcript variant 1 were identified in ovarian cancer and control ovaries. By contrast, in both groups the expression levels of SDF-1 transcript variants 3 and 4 were extremely low. Furthermore, SDF-1 variant 1 levels were notably higher in epithelial ovarian cancer than in control ovaries, while data for the remaining transcripts were similar in both groups. CXCR4 transcript variant 2 and CXCR7 expression levels in normal and neoplastic ovaries were similar. In both groups, CXCR4 transcript variant 2 was not detected. Plasma SDF-1? levels were notably higher in females with epithelial ovarian cancer than in the control ovaries. Elevated levels of blood SDF-1? were found prior to surgery, 6 days after surgery and following completion of the first chemotherapy course. These increases were independent of the type of epithelial ovarian cancer. Our results suggest that the expression of SDF-1 and the genes controlling alternative splicing are elevated in epithelial ovarian cancer, leading to an increased formation of SDF-1 variant 1. Elevated plasma SDF-1? levels in epithelial ovarian cancer patients are not associated with the presence of tumors and/or metastases, however reflect a general response to the disease.

SUBMITTER: Jaszczynska-Nowinka K 

PROVIDER: S-EPMC3997724 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Expression of <i>SDF-1</i> and <i>CXCR4</i> transcript variants and <i>CXCR7</i> in epithelial ovarian cancer.

Jaszczynska-Nowinka Karolina K   Rucinski Marcin M   Ziolkowska Agnieszka A   Markowska Anna A   Malendowicz Ludwik K LK  

Oncology letters 20140220 5


Chemokine stromal cell-derived factor-1 (SDF-1) and its receptors, CXCR4 and CXCR7, have been implicated in epithelial ovarian cancer progression and metastasis. However, limited data are available on the expression levels of <i>SDF-1</i> and <i>CXCR4</i> variants and <i>CXCR7</i> in human epithelial ovarian cancer. The present study aimed to characterize the expression pattern and levels of <i>SDF-1</i>, <i>CXCR4</i> and <i>CXCR7</i> in normal human ovaries and epithelial ovarian cancer. The ex  ...[more]

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