Single cell genome analysis of an uncultured heterotrophic stramenopile.
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ABSTRACT:
SUBMITTER: Roy RS
PROVIDER: S-EPMC3998028 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Publications
Single cell genome analysis of an uncultured heterotrophic stramenopile.
Roy Rajat S RS Price Dana C DC Schliep Alexander A Cai Guohong G Korobeynikov Anton A Yoon Hwan Su HS Yang Eun Chan EC Bhattacharya Debashish D
Scientific reports 20140424
A broad swath of eukaryotic microbial biodiversity cannot be cultivated in the lab and is therefore inaccessible to conventional genome-wide comparative methods. One promising approach to study these lineages is single cell genomics (SCG), whereby an individual cell is captured from nature and genome data are produced from the amplified total DNA. Here we tested the efficacy of SCG to generate a draft genome assembly from a single sample, in this case a cell belonging to the broadly distributed ...[more]
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