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Genome-wide association study identifies novel loci associated with resistance to bovine tuberculosis.

ABSTRACT: Tuberculosis (TB) caused by Mycobacterium bovis is a re-emerging disease of livestock that is of major economic importance worldwide, as well as being a zoonotic risk. There is significant heritability for host resistance to bovine TB (bTB) in dairy cattle. To identify resistance loci for bTB, we undertook a genome-wide association study in female Holstein-Friesian cattle with 592 cases and 559 age-matched controls from case herds. Cases and controls were categorised into distinct phenotypes: skin test and lesion positive vs skin test negative on multiple occasions, respectively. These animals were genotyped with the Illumina BovineHD 700K BeadChip. Genome-wide rapid association using linear and logistic mixed models and regression (GRAMMAR), regional heritability mapping (RHM) and haplotype-sharing analysis identified two novel resistance loci that attained chromosome-wise significance, protein tyrosine phosphatase receptor T (PTPRT; P=4.8 × 10(-7)) and myosin IIIB (MYO3B; P=5.4 × 10(-6)). We estimated that 21% of the phenotypic variance in TB resistance could be explained by all of the informative single-nucleotide polymorphisms, of which the region encompassing the PTPRT gene accounted for 6.2% of the variance and a further 3.6% was associated with a putative copy number variant in MYO3B. The results from this study add to our understanding of variation in host control of infection and suggest that genetic marker-based selection for resistance to bTB has the potential to make a significant contribution to bTB control.

SUBMITTER: Bermingham ML

PROVIDER: S-EPMC3998787 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Genome-wide association study identifies novel loci associated with resistance to bovine tuberculosis.

Bermingham M L ML Bishop S C SC Woolliams J A JA Pong-Wong R R Allen A R AR McBride S H SH Ryder J J JJ Wright D M DM Skuce R A RA McDowell S Wj SW Glass E J EJ

Heredity 20140205 5

Tuberculosis (TB) caused by Mycobacterium bovis is a re-emerging disease of livestock that is of major economic importance worldwide, as well as being a zoonotic risk. There is significant heritability for host resistance to bovine TB (bTB) in dairy cattle. To identify resistance loci for bTB, we undertook a genome-wide association study in female Holstein-Friesian cattle with 592 cases and 559 age-matched controls from case herds. Cases and controls were categorised into distinct phenotypes: sk ...[more]

PMID: 24496092

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Genome-wide association study identifies novel loci associated with resistance to bovine tuberculosis.

Publications

Genome-wide association study identifies novel loci associated with resistance to bovine tuberculosis.

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