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Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry.


ABSTRACT: A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative.

SUBMITTER: Hughes AJ 

PROVIDER: S-EPMC3999232 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Investigating the reactivities of a polyketide synthase module through fluorescent click chemistry.

Hughes Amanda Jane AJ   Tibby Matthew R MR   Wagner Drew T DT   Brantley Johnathan N JN   Keatinge-Clay Adrian T AT  

Chemical communications (Cambridge, England) 20131106 40


A method for monitoring in vitro polyketide synthesis has been developed whereby nonchromophoric polyketide products are made brightly fluorescent in a simple, rapid, inexpensive, and bioorthogonal manner through CuAAC with a sulforhodamine B azide derivative. ...[more]

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