Dataset Information

Cross-sectional study of the relationship of peripheral blood cell profiles with severity of infection by adenovirus type 55.

ABSTRACT:

Background

The immunologic profiles of patients with human adenovirus serotype 55 (HAdV-55) infections were characterized in subjects diagnosed with silent infections (n?=?30), minor infections (n?=?27), severe infections (n?=?34), and healthy controls (n?=?30) during a recent outbreak among Chinese military trainees.

Methods

Blood was sampled at the disease peak and four weeks later, and samples were analyzed to measure changes in leukocyte and platelet profiles in patients with different severities of disease. Differential lymphocyte subsets and cytokine profiles were measured by flow cytometry and Luminex xMAP®, and serum antibodies were analyzed by ELISA and immunofluorescence staining.

Results

Patients with severe HAdV infections had higher proportions of neutrophils and reduced levels of lymphocytes (p?ConclusionsPatients with different severities of disease due to HAdV-55 infection had significantly different immune responses. These data provide an initial step toward the identification of patients at risk for more severe disease and the development of treatments against HAdV-55 infection.

SUBMITTER: Chen WW

PROVIDER: S-EPMC4000060 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Publications

Cross-sectional study of the relationship of peripheral blood cell profiles with severity of infection by adenovirus type 55.

Chen Wei-Wei WW Nie Wei-Min WM Xu Wen W Xie Yang-Xin YX Tu Bo B Zhao Peng P Qin En-Qiang EQ Zhang Yun-Hui YH Zhang Xiu X Li Wen-Gang WG Zhou Zhi-Ping ZP Lv Ji-Yun JY Zhao Min M

BMC infectious diseases 20140319

<h4>Background</h4>The immunologic profiles of patients with human adenovirus serotype 55 (HAdV-55) infections were characterized in subjects diagnosed with silent infections (n = 30), minor infections (n = 27), severe infections (n = 34), and healthy controls (n = 30) during a recent outbreak among Chinese military trainees.<h4>Methods</h4>Blood was sampled at the disease peak and four weeks later, and samples were analyzed to measure changes in leukocyte and platelet profiles in patients with ...[more]

PMID: 24646014

Similar Datasets

Project description:Background Human adenovirus (HAdV) infection outbreak causes community-acquired pneumonia. Cellular immune dysfunction and hypercytokinemia play important roles in the pathogenesis of adenovirus respiratory infection. Some soluble factors in peripheral blood can assist in judging the virus-induced disease severity. The expression levels of inflammatory cytokines differ among patients with different disease severity. However, whether and how HAdV-7 infection influences the composition of blood immune cells and serum cytokine levels in patients at different disease stages, as well as the diagnosis values of these parameters, have rarely been intensively studied. We aimed to investigate lymphocytes profiles and cytokines levels in blood of patients at different disease stages upon human adenovirus type 7 (HAdV-7) infections, and explored the diagnosis values of the investigated parameters. Methods Patients from two outbreaks of HAdV-7 in military of China were categorized into upper respiratory infection (URI) group, common pneumonia (CP) group and severe pneumonia (SP) group according to disease severity. Peripheral blood samples were subjected to routine laboratory tests, while flow cytometry and ELISA were used to measure the lymphocyte subsets and cytokines in blood, respectively. The receiver operating characteristic (ROC) curves were performed to examine the diagnostic of these blood parameters. Results Signs of imbalanced lymphocytes composition and hypercytokinemia were observed in HAdV-7-infected patients. The percentages of CD3+ T cells and NK cells were significantly decreased along with the aggravation of the disease, particularly for NK cells and CD4+ T cells. The neutrophil to lymphocyte ratio (NLR) increased significantly in patients with more severe disease. In addition, the levels of serum CXCL10, IL-2 and TNF-α were positively correlated with disease severity, while reduced levels of IFN-γ and IL-10 were found in SP patients. Furthermore, analysis of ROC showed that multiple parameters including the percentage of blood CD3+ cells and serum CXCL10 level could predict the progression of HAdV-7 infection. Conclusion Imbalance of immune state with hypercytokinemia occurred during HAdV-7 infection. The percentages of blood immune cells such as CD3+ T cells and the levels of serum cytokines such as CXCL10 showed potential diagnosis values in HAdV-7 infection. Supplementary Information The online version contains supplementary material available at 10.1186/s12879-023-08132-z.

Dataset Information

Cross-sectional study of the relationship of peripheral blood cell profiles with severity of infection by adenovirus type 55.

Background

Methods

Results

Publications

Cross-sectional study of the relationship of peripheral blood cell profiles with severity of infection by adenovirus type 55.

Similar Datasets

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