Ontology highlight
ABSTRACT:
SUBMITTER: Jonkers I
PROVIDER: S-EPMC4001325 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
eLife 20140429
Production of mRNA depends critically on the rate of RNA polymerase II (Pol II) elongation. To dissect Pol II dynamics in mouse ES cells, we inhibited Pol II transcription at either initiation or promoter-proximal pause escape with Triptolide or Flavopiridol, and tracked Pol II kinetically using GRO-seq. Both inhibitors block transcription of more than 95% of genes, showing that pause escape, like initiation, is a ubiquitous and crucial step within the transcription cycle. Moreover, paused Pol I ...[more]