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Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.

ABSTRACT: The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by providing a carboxylic acid dimer partner in the form of a Glu residue.

SUBMITTER: Vasileiou C

PROVIDER: S-EPMC4004609 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

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Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay.

Vasileiou Chrysoula C Lee Kin Sing Stephen KS Crist Rachael M RM Vaezeslami Soheila S Goins Sarah M SM Geiger James H JH Borhan Babak B

Proteins 20090801 2

The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by pro ...[more]

PMID: 19156818

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