Dataset Information

Impact of endobronchial allergen provocation on macrophage phenotype in asthmatics.

ABSTRACT:

Background

The role of M2 polarized macrophages (M?) during the allergic airway inflammation has been discussed in various animal models. However, their presence and relevance during the chronic and acute phase of allergic airway inflammation in humans has not been fully elucidated so far. In the present study we phenotypically characterized macrophages with regard to M2 polarization in mice, a human in vitro and a human ex vivo model with primary lung cells after endobronchial provocation.

Results

Macrophages remained polarized beyond clearance of the acute allergic airway inflammation in mice. Alveolar macrophages of asthmatics revealed increased mRNA expression of CCL13, CCL17 and CLEC10A in response to allergen challenge as well as increased surface expression of CD86. Further, mRNA expression of CCL13, CCL17, and CLEC10A was increased in asthmatics at baseline compared to healthy subjects. The mRNA expression of CCL17 and CLEC10A correlated significantly with the degree of eosinophilia (each P ConclusionsThis study supports previous findings of M2 macrophage polarization in asthmatic subjects during the acute course of the allergic inflammation and provides evidence for their contribution to the Th2 inflammation.

SUBMITTER: Winkler C

PROVIDER: S-EPMC4007705 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Publications

Impact of endobronchial allergen provocation on macrophage phenotype in asthmatics.

Winkler Carla C Witte Lena L Moraw Natali N Faulenbach Conny C Müller Meike M Holz Olaf O Schaumann Frank F Hohlfeld Jens M JM

BMC immunology 20140310

<h4>Background</h4>The role of M2 polarized macrophages (MΦ) during the allergic airway inflammation has been discussed in various animal models. However, their presence and relevance during the chronic and acute phase of allergic airway inflammation in humans has not been fully elucidated so far. In the present study we phenotypically characterized macrophages with regard to M2 polarization in mice, a human in vitro and a human ex vivo model with primary lung cells after endobronchial provocati ...[more]

PMID: 24612750

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Dataset Information

Impact of endobronchial allergen provocation on macrophage phenotype in asthmatics.

Background

Results

Publications

Impact of endobronchial allergen provocation on macrophage phenotype in asthmatics.

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