Artificial three-dimensional niches deconstruct pancreas development in vitro.
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ABSTRACT:
SUBMITTER: Greggio C
PROVIDER: S-EPMC4007719 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Publications
Artificial three-dimensional niches deconstruct pancreas development in vitro.
Greggio Chiara C De Franceschi Filippo F Figueiredo-Larsen Manuel M Gobaa Samy S Ranga Adrian A Semb Henrik H Lutolf Matthias M Grapin-Botton Anne A
Development (Cambridge, England) 20131101 21
In the context of a cellular therapy for diabetes, methods for pancreatic progenitor expansion and subsequent differentiation into insulin-producing beta cells would be extremely valuable. Here we establish three-dimensional culture conditions in Matrigel that enable the efficient expansion of dissociated mouse embryonic pancreatic progenitors. By manipulating the medium composition we generate either hollow spheres, which are mainly composed of pancreatic progenitors, or complex organoids that ...[more]
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