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Fluorescent live-cell imaging of metabolically incorporated unnatural cyclopropene-mannosamine derivatives.

ABSTRACT: Sugar coated: We recently developed methylcyclopropenes as low-molecular-weight tetrazine coupling partners. Here, we demonstrate that methylcyclopropenes can meet the stringent steric demands required for metabolic imaging of unnatural mannosamines on live cells. Using sequential azide-alkyne chemistry, we also demonstrate multicolor imaging of two different metabolically incorporated unnatural sugars.

SUBMITTER: Cole CM

PROVIDER: S-EPMC4010072 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature

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Fluorescent live-cell imaging of metabolically incorporated unnatural cyclopropene-mannosamine derivatives.

Cole Christian M CM Yang Jun J Šečkutė Jolita J Devaraj Neal K NK

Chembiochem : a European journal of chemical biology 20130104 2

Sugar coated: We recently developed methylcyclopropenes as low-molecular-weight tetrazine coupling partners. Here, we demonstrate that methylcyclopropenes can meet the stringent steric demands required for metabolic imaging of unnatural mannosamines on live cells. Using sequential azide-alkyne chemistry, we also demonstrate multicolor imaging of two different metabolically incorporated unnatural sugars. ...[more]

PMID: 23292753

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