Ontology highlight
ABSTRACT:
SUBMITTER: Turner M
PROVIDER: S-EPMC4010539 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Plant signaling & behavior 20130515 8
We recently reported that hairpin (or stem-loop) priming is better-suited than polyA tailing to generate cDNA for plant microRNA qPCR. One major limitation of this method is the need to perform individual cDNA synthesis reactions for the reference gene and test miRNAs. Here, we report a novel fusion primer that allows multiplexed hairpin cDNA synthesis of the most-commonly used reference gene, nucleolar small RNA U6, together with test miRNAs. We also propose the use of miR1515 as a house keepin ...[more]