TALENs mediate efficient and heritable mutation of endogenous genes in the marine annelid Platynereis dumerilii.

ABSTRACT: Platynereis dumerilii is a marine polychaete and an established model system for studies of evolution and development. Platynereis is also a re-emerging model for studying the molecular basis of circalunar reproductive timing: a biological phenomenon observed in many marine species. While gene expression studies have provided new insight into patterns of gene regulation, a lack of reverse genetic tools has so far limited the depth of functional analyses in this species. To address this need, we established customized transcriptional activator-like effector nucleases (TALENs) as a tool to engineer targeted modifications in Platynereis genes. By adapting a workflow of TALEN construction protocols and mutation screening approaches for use in Platynereis, we engineered frameshift mutations in

SUBMITTER: Bannister S 

PROVIDER: S-EPMC4012502 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature