Membrane protein structure determination: back to the membrane.
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ABSTRACT:
SUBMITTER: Yao Y
PROVIDER: S-EPMC4012560 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Membrane protein structure determination: back to the membrane.
Yao Yong Y Ding Yi Y Tian Ye Y Opella Stanley J SJ Marassi Francesca M FM
Methods in molecular biology (Clifton, N.J.) 20130101
NMR spectroscopy enables the structures of membrane proteins to be determined in the native-like environment of the phospholipid bilayer membrane. This chapter outlines the methods for membrane protein structural studies using solid-state NMR spectroscopy with samples of membrane proteins incorporated in proteoliposomes or planar lipid bilayers. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from OmpX and A ...[more]
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