Sandwich assay for mixed-sequence recognition of double-stranded DNA: invader-based detection of targets specific to foodborne pathogens.
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ABSTRACT:
SUBMITTER: Denn B
PROVIDER: S-EPMC4013274 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Publications
Sandwich assay for mixed-sequence recognition of double-stranded DNA: invader-based detection of targets specific to foodborne pathogens.
Denn Benjamin B Karmakar Saswata S Guenther Dale C DC Hrdlicka Patrick J PJ
Chemical communications (Cambridge, England) 20131001 84
A 96-well plate sandwich assay based on Invader capture/signalling probes is used to recognize 28-mer mixed-sequence dsDNA targets specific to Salmonella enterica, Campylobacter jejuni, Escherichia coli. Targets are detected down to 20-55 pM concentration with excellent binding specificity. ...[more]
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