Unknown

Dataset Information

0

Cloning, expression and characterization of a novel thermophilic polygalacturonase from Caldicellulosiruptor bescii DSM 6725.


ABSTRACT: We cloned the gene ACM61449 from anaerobic, thermophilic Caldicellulosiruptor bescii, and expressed it in Escherichia coli origami (DE3). After purification through thermal treatment and Ni-NTA agarose column extraction, we characterized the properties of the recombinant protein (CbPelA). The optimal temperature and pH of the protein were 72 °C and 5.2, respectively. CbPelA demonstrated high thermal-stability, with a half-life of 14 h at 70 °C. CbPelA also showed very high activity for polygalacturonic acid (PGA), and released monogalacturonic acid as its sole product. The Vmax and Km of CbPelA were 384.6 U·mg?¹ and 0.31 mg·mL?¹, respectively. CbPelA was also able to hydrolyze methylated pectin (48% and 10% relative activity on 20%-34% and 85% methylated pectin, respectively). The high thermo-activity and methylated pectin hydrolization activity of CbPelA suggest that it has potential applications in the food and textile industry.

SUBMITTER: Chen Y 

PROVIDER: S-EPMC4013591 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Cloning, expression and characterization of a novel thermophilic polygalacturonase from Caldicellulosiruptor bescii DSM 6725.

Chen Yanyan Y   Sun Dejun D   Zhou Yulai Y   Liu Liping L   Han Weiwei W   Zheng Baisong B   Wang Zhi Z   Zhang Zuoming Z  

International journal of molecular sciences 20140403 4


We cloned the gene ACM61449 from anaerobic, thermophilic Caldicellulosiruptor bescii, and expressed it in Escherichia coli origami (DE3). After purification through thermal treatment and Ni-NTA agarose column extraction, we characterized the properties of the recombinant protein (CbPelA). The optimal temperature and pH of the protein were 72 °C and 5.2, respectively. CbPelA demonstrated high thermal-stability, with a half-life of 14 h at 70 °C. CbPelA also showed very high activity for polygalac  ...[more]

Similar Datasets

| S-EPMC3082886 | biostudies-literature
| S-EPMC5494628 | biostudies-literature
| S-EPMC4269323 | biostudies-literature
| S-EPMC6041484 | biostudies-literature
2017-11-28 | GSE107393 | GEO
| S-EPMC10019236 | biostudies-literature
| S-EPMC6500515 | biostudies-literature
| S-EPMC6597818 | biostudies-literature
| S-EPMC4992532 | biostudies-literature
| S-EPMC7071700 | biostudies-literature