Ontology highlight
ABSTRACT:
SUBMITTER: Chen Y
PROVIDER: S-EPMC4013591 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Chen Yanyan Y Sun Dejun D Zhou Yulai Y Liu Liping L Han Weiwei W Zheng Baisong B Wang Zhi Z Zhang Zuoming Z
International journal of molecular sciences 20140403 4
We cloned the gene ACM61449 from anaerobic, thermophilic Caldicellulosiruptor bescii, and expressed it in Escherichia coli origami (DE3). After purification through thermal treatment and Ni-NTA agarose column extraction, we characterized the properties of the recombinant protein (CbPelA). The optimal temperature and pH of the protein were 72 °C and 5.2, respectively. CbPelA demonstrated high thermal-stability, with a half-life of 14 h at 70 °C. CbPelA also showed very high activity for polygalac ...[more]