The yeast and human FACT chromatin-reorganizing complexes solve R-loop-mediated transcription-replication conflicts.

ABSTRACT: FACT (facilitates chromatin transcription) is a chromatin-reorganizing complex that swaps nucleosomes around the RNA polymerase during transcription elongation and has a role in replication that is not fully understood yet. Here we show that recombination factors are required for the survival of yeast FACT mutants, consistent with an accumulation of DNA breaks that we detected by Rad52 foci and transcription-dependent hyperrecombination. Breaks also accumulate in FACT-depleted human cells, as shown by γH2AX foci and single-cell electrophoresis. Furthermore, FACT-deficient yeast and human cells show replication impairment, which in yeast we demonstrate by ChIP-chip (chromatin immunoprecipitation [ChIP] coupled with microarray analysis) of Rrm3 to occur genome-wide but preferentially at high

SUBMITTER: Herrera-Moyano E 

PROVIDER: S-EPMC4015491 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature