Making ends meet: chemically mediated circularization of recombinant proteins.
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ABSTRACT:
SUBMITTER: Cowper B
PROVIDER: S-EPMC4016753 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
Making ends meet: chemically mediated circularization of recombinant proteins.
Cowper Ben B Craik David J DJ Macmillan Derek D
Chembiochem : a European journal of chemical biology 20130404 7
A selective N→S acyl transfer reaction facilitates semi-synthesis of the plant cyclotide kalata B1 from a linear precursor peptide of bacterial origin, through simple appendage of N-terminal cysteine and a thiol-labile C-terminal Gly-Cys motif. This constitutes the first synthesis of a ribosomally derived circular miniprotein, without recourse to protein splicing elements. ...[more]
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