Ontology highlight
ABSTRACT:
SUBMITTER: Shirayama M
PROVIDER: S-EPMC4017897 | biostudies-literature | 2014 Apr
REPOSITORIES: biostudies-literature
Shirayama Masaki M Stanney William W Gu Weifeng W Seth Meetu M Mello Craig C CC
Current biology : CB 20140327 8
Argonaute (AGO) proteins are key nuclease effectors of RNAi. Although purified AGOs can mediate a single round of target RNA cleavage in vitro, accessory factors are required for small interfering RNA (siRNA) loading and to achieve multiple-target turnover. To identify AGO cofactors, we immunoprecipitated the C. elegans AGO WAGO-1, which engages amplified small RNAs during RNAi. These studies identified a robust association between WAGO-1 and a conserved Vasa ATPase-related protein RDE-12. rde-1 ...[more]