Macrophage migration inhibitory factor: association of -794 CATT5-8 and -173 G>C polymorphisms with TNF-? in systemic lupus erythematosus.

ABSTRACT: Macrophage migration inhibitory factor (MIF) is an upstream immunoregulatory cytokine associated with the pathogenesis of autoimmune inflammatory diseases. There is evidence that MIF functions in a positive feedback loop with TNF-? that could perpetuate the inflammatory process in systemic lupus erythematosus (SLE). In this case-control study we investigated whether commonly occurring functional MIF polymorphisms are associated with SLE as well as with MIF and TNF-? serum levels in a Mexican-Mestizo population. Genotyping of the -794 CATT5-8 (rs5844572) and -173 G>C (rs755622) MIF polymorphisms was performed by PCR and PCR-RFLP, respectively in 186 SLE patients and 200 healthy subjects. MIF and TNF-? serum levels were determined by ELISA. A significant increase of MIF and TNF-? levels was found in SLE patients. According to a genetic model, we found a significant association of genotypes carrying the -794 CATT7 and -173(?)C risk alleles with susceptibility to SLE and with a significant increase of TNF-?. In conclusion, MIF gene polymorphisms are associated with SLE susceptibility and with an increase of TNF-? serum levels in a Mexican-Mestizo population.

SUBMITTER: De la Cruz-Mosso U 

PROVIDER: S-EPMC4017948 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature