Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions.
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ABSTRACT:
SUBMITTER: Park M
PROVIDER: S-EPMC4018249 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Publications
Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions.
Park Miso M Sun Qing Q Liu Fang F DeLisa Matthew P MP Chen Wilfred W
PloS one 20140512 5
The systematic organization of enzymes is a key feature for the efficient operation of cascade reactions in nature. Here, we demonstrate a facile method to create nanoscale enzyme cascades by using engineered bacterial outer membrane vesicles (OMVs) that are spheroid nanoparticles (roughly 50 nm in diameter) produced by Gram-negative bacteria during all phases of growth. By taking advantage of the fact that OMVs naturally contain proteins found in the outer cell membrane, we displayed a trivalen ...[more]
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