Ontology highlight
ABSTRACT:
SUBMITTER: Hur KH
PROVIDER: S-EPMC4018325 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Hur Kwang-Ho KH Macdonald Patrick J PJ Berk Serkan S Angert C Isaac CI Chen Yan Y Mueller Joachim D JD
PloS one 20140512 5
The brightness of fluorescently labeled proteins provides an excellent marker for identifying protein interactions in living cells. Quantitative interpretation of brightness, however, hinges on a detailed understanding of the processes that affect the signal fluctuation of the fluorescent label. Here, we focus on the cumulative influence of photobleaching on brightness measurements in cells. Photobleaching within the finite volume of the cell leads to a depletion of the population of fluorescent ...[more]