Analysis of the benzene oxide-DNA adduct 7-phenylguanine by liquid chromatography-nanoelectrospray ionization-high resolution tandem mass spectrometry-parallel reaction monitoring: application to DNA from exposed mice and humans.

ABSTRACT: Benzene oxide, the initial metabolite of the human carcinogen benzene, reacts with DNA producing 7-phenylguanine (7-PhG) and other products. We developed a highly sensitive liquid chromatography-nanoelectrospray ionization-high resolution tandem mass spectrometry-parallel reaction monitoring method for the analysis of 7-PhG in DNA. Accuracy and precision of the method were established and the detection limit was about 8amol of 7-PhG injected on the column and less than 1 adduct per 10(9) nucleotides in DNA. 7-PhG was detected in calf thymus DNA reacted with 1μM to 10mM benzene oxide. The method was applied for the analysis of DNA isolated from bone marrow, lung, and liver of B6C3F1 mice treated by gavage with 50mg/kg benzene in corn oil 5 times weekly for 4weeks. 7-PhG was not detected in

SUBMITTER: Zarth AT 

PROVIDER: S-EPMC4022132 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature