A bead-based multiplex assay for the detection of DNA viruses infecting laboratory rodents.

ABSTRACT: The Federation of European Laboratory Animal Science Association (FELASA) recommends screening of laboratory rodents and biological materials for a broad variety of bacterial agents, viruses, and parasites. Methods commonly used to date for pathogen detection are neither cost-effective nor time- and animal-efficient or uniform. However, an infection even if silent alters experimental results through changing the animals' physiology and increases inter-individual variability. As a consequence higher numbers of animals and experiments are needed for valid and significant results. We developed a novel high-throughput multiplex assay, called rodent DNA virus finder (rDVF) for the simultaneous identification of 24 DNA viruses infecting mice and rats. We detected all 24 DNA viruses with high spe

SUBMITTER: Hofler D 

PROVIDER: S-EPMC4023972 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature