Ontology highlight
ABSTRACT:
SUBMITTER: Liu H
PROVIDER: S-EPMC4024384 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Cell stem cell 20140213 3
Recent advances in gene editing technology have introduced the potential for application of mutagenesis approaches in nonhuman primates to model human development and disease. Here we report successful TALEN-mediated mutagenesis of an X-linked, Rett syndrome (RTT) gene, methyl-CpG binding protein 2 (MECP2), in both rhesus and cynomolgus monkeys. Microinjection of MECP2-targeting TALEN plasmids into rhesus and cynomolgus zygotes leads to effective gene editing of MECP2 with no detected off-target ...[more]