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Scalable production of highly sensitive nanosensors based on graphene functionalized with a designed G protein-coupled receptor.

ABSTRACT: We have developed a novel, all-electronic biosensor for opioids that consists of an engineered ?-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transistors with average mobility of ?1500 cm(2) V(-1) s(-1) and yield exceeding 98%. The biosensor exhibits high sensitivity and selectivity for the target naltrexone, an opioid receptor antagonist, with a detection limit of 10 pg/mL.

SUBMITTER: Lerner MB

PROVIDER: S-EPMC4025580 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Scalable production of highly sensitive nanosensors based on graphene functionalized with a designed G protein-coupled receptor.

Lerner Mitchell B MB Matsunaga Felipe F Han Gang Hee GH Hong Sung Ju SJ Xi Jin J Crook Alexander A Perez-Aguilar Jose Manuel JM Park Yung Woo YW Saven Jeffery G JG Liu Renyu R Johnson A T Charlie AT

Nano letters 20140421 5

We have developed a novel, all-electronic biosensor for opioids that consists of an engineered μ-opioid receptor protein, with high binding affinity for opioids, chemically bonded to a graphene field-effect transistor to read out ligand binding. A variant of the receptor protein that provided chemical recognition was computationally redesigned to enhance its solubility and stability in an aqueous environment. A shadow mask process was developed to fabricate arrays of hundreds of graphene transis ...[more]

PMID: 24742304

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