Proposed Bioactive Conformations of Opiorphin, an Endogenous Dual APN/NEP Inhibitor.

ABSTRACT: The conformational profiles for the endogenous peptide Opiorphin and a set of seven analogues exhibiting different inhibitory activities toward human aminopeptidase N (hAPN) and human neprilysin (hNEP) were independently computed to deduce a bioactive conformation that Opiorphin may adopt when binding these two enzymes. The conformational space was thoroughly sampled using an iterative simulated annealing protocol, and a library of low-energy conformers was generated for each peptide. Bioactive Opiorphin conformations fitting our experimental structure-activity relationship data were identified for hAPN and hNEP using computational pairwise comparisons between each of the unique low-energy conformations of Opiorphin and its analogues. The obtained results provide a structural explanation f

SUBMITTER: Pinto M 

PROVIDER: S-EPMC4025773 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature