Unknown

Dataset Information

0

Chronic expression of PPAR-delta by oligodendrocyte lineage cells in the injured rat spinal cord.


ABSTRACT: The transcription factor peroxisome proliferator-activated receptor (PPAR)-delta promotes oligodendrocyte differentiation and myelin formation in vitro and is prevalent throughout the brain and spinal cord. Its expression after injury, however, has not been examined. Thus, we used a spinal contusion model to examine the spatiotemporal expression of PPAR-delta in naïve and injured spinal cords from adult rats. As previously reported, PPAR-delta was expressed by neurons and oligodendrocytes in uninjured spinal cords; PPAR-delta was also detected in NG2 cells (potential oligodendrocyte progenitors) within the white matter and gray matter. After spinal cord injury (SCI), PPAR-delta mRNA and protein were present early and increased over time. Overall PPAR-delta+ cell numbers declined at 1 day post injury (dpi), likely reflecting neuron loss, and then rose through 14 dpi. A large proportion of NG2 cells expressed PPAR-delta after SCI, especially along lesion borders. PPAR-delta+ NG2 cell numbers were significantly higher than naive by 7 dpi and remained elevated through at least 28 dpi. PPAR-delta+ oligodendrocyte numbers declined at 1 dpi and then increased over time such that >20% of oligodendrocytes expressed PPAR-delta after SCI compared with approximately 10% in uninjured tissue. The most prominent increase in PPAR-delta+ oligodendrocytes was along lesion borders where at least a portion of newly generated oligodendrocytes (bromodeoxyuridine+) were PPAR-delta+. Consistent with its role in cellular differentiation, the early rise in PPAR-delta+ NG2 cells followed by an increase in new PPAR-delta+ oligodendrocytes suggests that this transcription factor may be involved in the robust oligodendrogenesis detected previously along SCI lesion borders.

SUBMITTER: Almad A 

PROVIDER: S-EPMC4026285 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Chronic expression of PPAR-delta by oligodendrocyte lineage cells in the injured rat spinal cord.

Almad Akshata A   McTigue Dana M DM  

The Journal of comparative neurology 20100301 6


The transcription factor peroxisome proliferator-activated receptor (PPAR)-delta promotes oligodendrocyte differentiation and myelin formation in vitro and is prevalent throughout the brain and spinal cord. Its expression after injury, however, has not been examined. Thus, we used a spinal contusion model to examine the spatiotemporal expression of PPAR-delta in naïve and injured spinal cords from adult rats. As previously reported, PPAR-delta was expressed by neurons and oligodendrocytes in uni  ...[more]

Similar Datasets

| S-EPMC4899531 | biostudies-literature
| S-EPMC9813964 | biostudies-literature
| S-EPMC6407839 | biostudies-literature
| S-EPMC7475673 | biostudies-literature
| S-EPMC6628227 | biostudies-literature
| S-EPMC5637237 | biostudies-literature
| S-EPMC10538588 | biostudies-literature
| S-EPMC3101831 | biostudies-literature
| S-EPMC6293371 | biostudies-literature
| S-EPMC5312572 | biostudies-literature