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New Peptide-based antimicrobials for tackling drug resistance in bacteria: single-cell fluorescence imaging.

ABSTRACT: New peptide molecules with metal binding abilities proved to be active against multidrug resistant clinical isolates. One of them labeled with a dansylated lysine has been imaged inside single-multidrug resistant bacteria cells by deep ultraviolet fluorescence, showing a heterogeneous subcellular localization. The fluorescence intensity is clearly related to the accumulation of the drug inside the bacteria, being dependent both on its concentration and on the incubation time with cells.

SUBMITTER: Pages JM

PROVIDER: S-EPMC4027424 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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New Peptide-based antimicrobials for tackling drug resistance in bacteria: single-cell fluorescence imaging.

Pagès Jean-Marie JM Kascàkovà Slavka S Maigre Laure L Allam Anas A Alimi Mickael M Chevalier Jacqueline J Galardon Erwan E Réfrégiers Matthieu M Artaud Isabelle I

ACS medicinal chemistry letters 20130507 6

New peptide molecules with metal binding abilities proved to be active against multidrug resistant clinical isolates. One of them labeled with a dansylated lysine has been imaged inside single-multidrug resistant bacteria cells by deep ultraviolet fluorescence, showing a heterogeneous subcellular localization. The fluorescence intensity is clearly related to the accumulation of the drug inside the bacteria, being dependent both on its concentration and on the incubation time with cells. ...[more]

PMID: 24900708

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