Dataset Information

Metal impurities cause false positives in high-throughput screening campaigns.

ABSTRACT: Organic impurities in compound libraries are known to often cause false-positive signals in screening campaigns for new leads, but organic impurities do not fully account for all false-positive results. We discovered inorganic impurities in our screening library that can also cause positive signals for a variety of targets and/or readout systems, including biochemical and biosensor assays. We investigated in depth the example of zinc for a specific project and in retrospect in various HTS screens at Roche and propose a straightforward counter screen using the chelator TPEN to rule out inhibition caused by zinc.

SUBMITTER: Hermann JC

PROVIDER: S-EPMC4027514 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Metal impurities cause false positives in high-throughput screening campaigns.

Hermann Johannes C JC Chen Yingsi Y Wartchow Charles C Menke John J Gao Lin L Gleason Shelley K SK Haynes Nancy-Ellen NE Scott Nathan N Petersen Ann A Gabriel Stephen S Vu Binh B George Kelly M KM Narayanan Arjun A Li Shirley H SH Qian Hong H Beatini Nanda N Niu Linghao L Gan Qing-Fen QF

ACS medicinal chemistry letters 20121212 2

Organic impurities in compound libraries are known to often cause false-positive signals in screening campaigns for new leads, but organic impurities do not fully account for all false-positive results. We discovered inorganic impurities in our screening library that can also cause positive signals for a variety of targets and/or readout systems, including biochemical and biosensor assays. We investigated in depth the example of zinc for a specific project and in retrospect in various HTS screen ...[more]

PMID: 24900642

Dataset Information

Metal impurities cause false positives in high-throughput screening campaigns.

Publications

Metal impurities cause false positives in high-throughput screening campaigns.

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