Metal impurities cause false positives in high-throughput screening campaigns.
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ABSTRACT:
SUBMITTER: Hermann JC
PROVIDER: S-EPMC4027514 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Publications
Metal impurities cause false positives in high-throughput screening campaigns.
Hermann Johannes C JC Chen Yingsi Y Wartchow Charles C Menke John J Gao Lin L Gleason Shelley K SK Haynes Nancy-Ellen NE Scott Nathan N Petersen Ann A Gabriel Stephen S Vu Binh B George Kelly M KM Narayanan Arjun A Li Shirley H SH Qian Hong H Beatini Nanda N Niu Linghao L Gan Qing-Fen QF
ACS medicinal chemistry letters 20121212 2
Organic impurities in compound libraries are known to often cause false-positive signals in screening campaigns for new leads, but organic impurities do not fully account for all false-positive results. We discovered inorganic impurities in our screening library that can also cause positive signals for a variety of targets and/or readout systems, including biochemical and biosensor assays. We investigated in depth the example of zinc for a specific project and in retrospect in various HTS screen ...[more]
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