Regulation of angiotensin II type 1 receptor expression in ovarian cancer: a potential role for BRCA1.

ABSTRACT:

Background

Both BRCA1 and angiotensin II type 1 receptor (AGTR1) play a critical role in ovarian cancer progression. However, the crosstalk between BRCA1 and AGTR1 signaling pathways remains largely unknown.

Methods

BRCA1 promoter methylation was analyzed by bisulfite sequence using primers focused on the core promoter region. Expression levels of BRCA1 and AGTR1 were assessed by immunohistochemistry and real-time PCR. Regression analysis was used to examine the possible relationship between BRCA1 and AGTR1 protein levels. Knockdown or overexpression of BRCA1 was achieved by using a lentiviral vector in 293 T cells and SKOV3 ovarian carcinoma cells, and primary non-mutated and BRCA1-mutated ovarian cancer cells.

Results

BRCA1 dysfunction (BRCA1 mutation or hypermethyl

SUBMITTER: Bi FF 

PROVIDER: S-EPMC4029559 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature