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Methods for multiplex template sampling in digital PCR assays.

ABSTRACT: The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing a 10-fold concentration enhancement while preserving assay precision.

SUBMITTER: Petriv OI 

PROVIDER: S-EPMC4031183 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Methods for multiplex template sampling in digital PCR assays.

Petriv Oleh I OI   Heyries Kevin A KA   VanInsberghe Michael M   Walker David D   Hansen Carl L CL  

PloS one 20140522 5

The efficient use of digital PCR (dPCR) for precision copy number analysis requires high concentrations of target molecules that may be difficult or impossible to obtain from clinical samples. To solve this problem we present a strategy, called Multiplex Template Sampling (MTS), that effectively increases template concentrations by detecting multiple regions of fragmented target molecules. Three alternative assay approaches are presented for implementing MTS analysis of chromosome 21, providing  ...[more]

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