Ontology highlight
ABSTRACT:
SUBMITTER: Rafiee M
PROVIDER: S-EPMC4032159 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Rafiee Mohammad M Jahangiri-Rad Mahsa M Hajjaran Homa H Mesdaghinia Alireza A Hajaghazadeh Mohammad M
Journal of environmental health science & engineering 20140509
Legionella spp. are important waterborne pathogens that are normally transmitted through aerosols. The present work was conducted to investigate the presence of Legionella spp. and its common species in hospital water supplies. Considering the limitations of culture method, polymerase chain reaction (PCR) assays were developed to detect the gene 16S rRNA irrespective of the bacterial serotype. Four well-established DNA extraction protocols (freeze & thaw and phenol-chloroform as two manual proto ...[more]