Glycoengineering of interferon-? 1a improves its biophysical and pharmacokinetic properties.
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ABSTRACT:
SUBMITTER: Song K
PROVIDER: S-EPMC4032242 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
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Glycoengineering of interferon-β 1a improves its biophysical and pharmacokinetic properties.
Song Kyoung K Yoon In-Soo IS Kim Nam Ah NA Kim Dong-Hwan DH Lee Jongmin J Lee Hee Jung HJ Lee Saehyung S Choi Sunghyun S Choi Min-Koo MK Kim Ha Hyung HH Jeong Seong Hoon SH Son Woo Sung WS Kim Dae-Duk DD Shin Young Kee YK
PloS one 20140523 5
The purpose of this study was to develop a biobetter version of recombinant human interferon-β 1a (rhIFN-β 1a) to improve its biophysical properties, such as aggregation, production and stability, and pharmacokinetic properties without jeopardizing its activity. To achieve this, we introduced additional glycosylation into rhIFN-β 1a via site-directed mutagenesis. Glycoengineering of rhIFN-β 1a resulted in a new molecular entity, termed R27T, which was defined as a rhIFN-β mutein with two N-glyco ...[more]
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